Interactions of bovine neurophysin II with oxytocin and (8-lysine) vasopressin. High resolution proton nuclear magnetic resonance spectroscopy studies.
نویسندگان
چکیده
From the Labor&ire de Biochimie, U.E.R. 58, Universit6 Paris VI Paris, France; the Service de Biochimie C,E.N. Saclay, 91190 Gif-sur-Yvette France; und the Reproduction Research Branch, National Institute oj Child Health and Human Developmeni and the Laboratory of Chemical Biology, National Institutes of Arthritis Metabolic, and Digestive Diseases, National Institutes of Health, Bethesda, Maryland 20014
منابع مشابه
Interactions of bovine neurophysins with neurohypophyseal hormones. On the role of tyrosine-49.
Reaction of tetranitromethane with the lone tyrosine residue of bovine neurophysin I and II, tyrosine-49, gave nitro derivatives of these proteins which were obtained in a highly purified form by preparative electrophoresis. Equilibrium dialysis experiments indicated clearly that oxytocin binding remained essentially unaffected by the chemical modification of tyrosine-49. However, in the case o...
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The assignments and partial conformational analysis of the 220-MHz (1)H nuclear magnetic resonance spectrum of the neurohypophyseal hormones arginine vasopressin and arginine vasotocin in uniformaly deuterated dimethylsulfoxide are reported. The chemical shifts, vicinal coupling constants, temperature coefficients of chemical shifts, and proton-deuterium exchange rates for protons of arginine v...
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1. The native hormone-binding proteins, neurophysin-I and -II, have been isolated from acetone-desiccated bovine pituitary posterior lobes. 2. Neurophysin-I and -II are present in approximately equal quantities in the tissue and are localized in the neurosecretory granules. 3. The apparent molecular weight, determined by equilibrium sedimentation of neurophysin-I, was 19000 and that of neurophy...
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The effects of hormone binding on the reversible monomer in equilibrium dimer equilibrium of bovine neurophysins I or II in solution have been studied by sedimentation equilibrium measurements performed in conjunction with equilibrium dialysis experiments. Under normal solution conditions saturating amounts of oxytocin displace the neurophysin dimerization equilibrium toward the associated form...
متن کاملImmunological identification of a common precursor to arginine vasopressin and neurophysin II synthesized by in vitro translation of bovine hypothalamic mRNA.
mRNA from membrane-bound polysomes of bovine hypothalamus was translated in an mRNA-dependent cell-free system from reticulocyte lysate or wheat germ. The translation products were identified by immunoprecipitation with antibodies to either neurophysin II or arginine vasopressin followed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. An immunoreactive polypeptide was obtained wit...
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 249 21 شماره
صفحات -
تاریخ انتشار 1974